Effect of Anyama Green Clay on Inflammation: Evaluation of Hematological Parameters in Wistar Rats
Effet de l'Argile Verte d'Anyama Sur l'Inflammation : Évaluation des Paramètres Hématologiques Chez le Rat Wistar
DOI:
https://doi.org/10.5281/zenodo.19591339%20Keywords:
Inflammation, green clay, hematology, bone healing, Wistar ratAbstract
RÉSUMÉ
Introduction. L’argile avec ses propriétés bactéricide et antitoxine [1], présente un atout pour la médecine dentaire moderne. L’objectif de cette étude était d’analyser l’impact de l’argile verte d’Anyama insérée en site osseux, sur l’inflammation à travers le dosage des paramètres hématologiques du rat Wistar. Méthodologie. Trente-six rats Wistar âgés de 10 mois ont été soumis à la création de défauts osseux au niveau du diastème post-canin supérieur gauche, après anesthésie à la kétamine ou à l'éther, afin d'évaluer l'impact de l'argile verte d'Anyama sur la cicatrisation. Les animaux ont été répartis en huit lots expérimentaux recevant 100 mg d'argile sous forme pâteuse ou sèche à différents pH, ainsi que deux lots témoins. Le suivi biologique a reposé sur des prélèvements sanguins effectués à J0, J15 et J30 pour l'analyse de paramètres hématologiques réalisés à l'Institut Pasteur. Résultats. Les valeurs des paramètres hématologiques à J0 correspondent aux données des rats Wistar sans exposition de l’argile verte d’Anyama. A J15, on constate que le taux de globule blanc (GB) était élevé chez les lots traités par rapport aux témoins et une persistance du taux élevé de GB à J30 du lot MPN chez les mâles. Pour les autres paramètres hématologiques, on note une légère neutrophilie et le lot traité à l’argile sèche à pH basique (MSB) était le seul à avoir un taux de monocyte bas à J15. Conclusion. L’argile verte d’Anyama a pH basique accélère la guérison osseuse avec la réduction de la durée de l’inflammation matérialisée par un taux bas de lymphocyte et monocyte à J30 (p-value ˂ 0.05).
ABSTRACT
Introduction. With its bactericidal and antitoxin properties, clay represents an asset for modern dentistry. The objective of this study was to analyze the impact of Anyama green clay inserted into bone sites on inflammation by measuring hematological parameters in Wistar rats. Methodology. Thirty-six 10-month-old Wistar rats underwent the creation of bone defects at the level of the upper left post-canine diastema, following anesthesia with ketamine or ether, to evaluate the impact of Anyama green clay on healing. The animals were divided into eight experimental groups receiving 100 mg of clay in either paste or dry form at different pH levels, along with two control groups. Biological monitoring was based on blood samples taken on day 0, day 15, and day 30 for the analysis of hematological parameters conducted at the Institut Pasteur. Results. The hematological parameter values at day 0 correspond to the data for Wistar rats not exposed to Anyama green clay. At day 15, the white blood cell (WBC) count was higher in the treated groups than in the controls, and the WBC count remained high at day 30 in the MPN group in males. For the other hematological parameters, a slight neutrophilia was noted, and the group treated with dry clay at basic pH (MSB) was the only one to have a low monocyte count on day 15. Conclusion. Anyama green clay, which has a basic pH, accelerates bone healing by reducing the duration of inflammation, as evidenced by low lymphocyte and monocyte counts on day 30 (p-value ˂ 0.05).
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